› 3d50

X-ray diffraction
1.79Å resolution

GOLGI MANNOSIDASE II complex with N-octyl-6-epi-valienamine

Released:
DOI: 10.2210/pdb3d50/pdb
PDB entry 3d50 coloured by chain, front view.
PDB entry 3d50 coloured by chain, side view.
PDB entry 3d50 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural analysis of Golgi alpha-mannosidase II inhibitors identified from a focused glycosidase inhibitor screen.
Kuntz DA, Tarling CA, Withers SG, Rose DR
Biochemistry 47 10058-68 (2008)
PMID: 18759458

Function and Biology Details

Reaction catalysed: 
Man(5)GlcNAc(3)-[protein] + 2 H(2)O = Man(3)GlcNAc(3)-[protein] + 2 alpha-D-mannopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

4 bound ligands:
Ligand NAG 1 x NAG
Ligand ZN 1 x ZN
Ligand OEV 1 x OEV
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 68.842Å b: 109.964Å c: 138.058Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.156 0.187
Expression system: Drosophila melanogaster

Quick links

Citations

2 review citations

Recent developments in design and synthesis of bicyclic azasugars, carbasugars and related molecules as glycosidase inhibitors.
Lahiri et al. (2013)
1 more

3 mentions without citation

The molecular basis of inhibition of Golgi alpha-mannosidase II by mannostatin A.
Kuntz et al. (2009)
2 more