› 3d52

X-ray diffraction
1.6Å resolution

GOLGI MANNOSIDASE II complex with an N-aryl carbamate derivative of gluco-hydroxyiminolactam

Released:
DOI: 10.2210/pdb3d52/pdb
PDB entry 3d52 coloured by chain, front view.
PDB entry 3d52 coloured by chain, side view.
PDB entry 3d52 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural analysis of Golgi alpha-mannosidase II inhibitors identified from a focused glycosidase inhibitor screen.
Kuntz DA, Tarling CA, Withers SG, Rose DR
Biochemistry 47 10058-68 (2008)
PMID: 18759458

Function and Biology Details

Reaction catalysed: 
Man(5)GlcNAc(3)-[protein] + 2 H(2)O = Man(3)GlcNAc(3)-[protein] + 2 alpha-D-mannopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand MRD 1 x MRD
Ligand GHR 1 x GHR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 68.579Å b: 107.912Å c: 137.062Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.24
Expression system: Drosophila melanogaster

Quick links

Citations

2 review citations

Recent developments in design and synthesis of bicyclic azasugars, carbasugars and related molecules as glycosidase inhibitors.
Lahiri et al. (2013)
1 more

2 mentions without citation

The molecular basis of inhibition of Golgi alpha-mannosidase II by mannostatin A.
Kuntz et al. (2009)
1 more