› 3d5t

X-ray diffraction
2.51Å resolution

Crystal structure of malate dehydrogenase from Burkholderia pseudomallei

Released:
DOI: 10.2210/pdb3d5t/pdb
PDB entry 3d5t coloured by chain, front view.
PDB entry 3d5t coloured by chain, side view.
PDB entry 3d5t coloured by chain, top view.
Source organism: Burkholderia pseudomallei 1710b
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174640 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 331 amino acids
Theoretical weight: 35.35 KDa
Source organism: Burkholderia pseudomallei 1710b
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JKE9 (Residues: 1-327; Coverage: 100%)
Gene names: BURPS1710b_A0795, mdh
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 59.387Å b: 79.608Å c: 288.874Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.241
Expression system: Escherichia coli

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