› 3d5v

X-ray diffraction
2.4Å resolution

Crystal structure of an activated (Thr->Asp) Polo-like kinase 1 (Plk1) catalytic domain.

Released:
DOI: 10.2210/pdb3d5v/pdb
PDB entry 3d5v coloured by chain, front view.
PDB entry 3d5v coloured by chain, side view.
PDB entry 3d5v coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structures of the wild-type and activated catalytic domains of Brachydanio rerio Polo-like kinase 1 (Plk1): changes in the active-site conformation and interactions with ligands.
Elling RA, Fucini RV, Romanowski MJ
Acta Crystallogr D Biol Crystallogr 64 909-18 (2008)
PMID: 18703838

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PLK Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 35.99 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6DRK7 (Residues: 1-312; Coverage: 52%)
Gene name: plk1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I23
Unit cell:
a: 134.767Å b: 134.767Å c: 134.767Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.234 0.269
Expression system: Escherichia coli

Quick links

Citations

2 review citations

On the molecular mechanisms of mitotic kinase activation.
Bayliss et al. (2012)
1 more

1 mention without citation

Structure of the Brachydanio rerio Polo-like kinase 1 (Plk1) catalytic domain in complex with an extended inhibitor targeting the adaptive pocket of the enzyme.
Elling et al. (2008)