› 3d7m

X-ray diffraction
2.9Å resolution

Crystal Structure of the G Protein Fast-Exchange Double Mutant I56C/Q333C

Released:
DOI: 10.2210/pdb3d7m/pdb
PDB entry 3d7m coloured by chain, front view.
PDB entry 3d7m coloured by chain, side view.
PDB entry 3d7m coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Helix dipole movement and conformational variability contribute to allosteric GDP release in Galphai subunits.
Preininger AM, Funk MA, Oldham WM, Meier SM, Johnston CA, Adhikary S, Kimple AJ, Siderovski DP, Hamm HE, Iverson TM
Biochemistry 48 2630-42 (2009)
PMID: 19222191

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.36 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 1-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ALF 1 x ALF
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P43212
Unit cell:
a: 79.659Å b: 79.659Å c: 114.587Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.249 0.294
Expression system: Escherichia coli

Quick links

Citations

4 review citations

A G protein-coupled receptor at work: the rhodopsin model.
Hofmann et al. (2009)
3 more

2 mentions without citation

Evaluating caveolin interactions: do proteins interact with the caveolin scaffolding domain through a widespread aromatic residue-rich motif?
Byrne et al. (2012)
1 more