› 3d7s

X-ray diffraction
2.8Å resolution

Crystal structure of Wild-Type E. Coli Asparate Transcarbamoylase at pH 8.5 at 2.80 A Resolution

Released:
DOI: 10.2210/pdb3d7s/pdb
PDB entry 3d7s coloured by chain, front view.
PDB entry 3d7s coloured by chain, side view.
PDB entry 3d7s coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The first high pH structure of Escherichia coli aspartate transcarbamoylase.
Stieglitz KA, Xia J, Kantrowitz ER
Proteins 74 318-27 (2009)
PMID: 18618694

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141554 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aspartate carbamoyltransferase catalytic subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 310 amino acids
Theoretical weight: 34.34 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A786 (Residues: 2-311; Coverage: 100%)
Gene names: JW4204, b4245, pyrB
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase
Aspartate carbamoyltransferase regulatory chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 153 amino acids
Theoretical weight: 17.14 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7F3 (Residues: 1-153; Coverage: 100%)
Gene names: JW4203, b4244, pyrI
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R3
Unit cell:
a: 129.68Å b: 129.68Å c: 198.58Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.235
Expression system: Escherichia coli

Quick links

Citations

2 review citations

From Genome to Structure and Back Again: A Family Portrait of the Transcarbamylases.
Shi et al. (2015)
1 more

5 mentions without citation

Binding leverage as a molecular basis for allosteric regulation.
Mitternacht et al. (2011)
4 more