› 3d8r

X-ray diffraction
2Å resolution

Thermus thermophilus Uroporphyrinogen III Synthase

Released:
DOI: 10.2210/pdb3d8r/pdb
PDB entry 3d8r coloured by chain, front view.
PDB entry 3d8r coloured by chain, side view.
PDB entry 3d8r coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structure and mechanistic implications of a uroporphyrinogen III synthase-product complex.
Schubert HL, Phillips JD, Heroux A, Hill CP
Biochemistry 47 8648-55 (2008)
PMID: 18651750

Function and Biology Details

Reaction catalysed: 
Hydroxymethylbilane = uroporphyrinogen III + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetrapyrrole biosynthesis uroporphyrinogen III synthase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 31.38 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72KM1 (Residues: 1-253; Coverage: 100%)
Gene name: TT_C0312
Sequence domains: Uroporphyrinogen-III synthase HemD
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P31
Unit cell:
a: 63.831Å b: 63.831Å c: 58.584Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.178 0.233
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structure and function of enzymes in heme biosynthesis.
Layer et al. (2010)
7 more

2 mentions without citation

Structure and mechanistic implications of a uroporphyrinogen III synthase-product complex.
Schubert et al. (2008)
1 more