› 3d8t

X-ray diffraction
1.6Å resolution

Thermus thermophilus Uroporphyrinogen III Synthase

Released:
DOI: 10.2210/pdb3d8t/pdb
PDB entry 3d8t coloured by chain, front view.
PDB entry 3d8t coloured by chain, side view.
PDB entry 3d8t coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structure and mechanistic implications of a uroporphyrinogen III synthase-product complex.
Schubert HL, Phillips JD, Heroux A, Hill CP
Biochemistry 47 8648-55 (2008)
PMID: 18651750

Function and Biology Details

Reaction catalysed: 
Hydroxymethylbilane = uroporphyrinogen III + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetrapyrrole biosynthesis uroporphyrinogen III synthase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 31.38 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72KM1 (Residues: 1-253; Coverage: 100%)
Gene name: TT_C0312
Sequence domains: Uroporphyrinogen-III synthase HemD
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand ACT 4 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 40.75Å b: 89.957Å c: 74.981Å
α: 90° β: 101.32° γ: 90°
R-values:
R R work R free
0.192 0.19 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Structure and function of enzymes in heme biosynthesis.
Layer et al. (2010)
7 more

1 mention without citation

Structure and mechanistic implications of a uroporphyrinogen III synthase-product complex.
Schubert et al. (2008)