› 3d97

X-ray diffraction
1.5Å resolution

Crystal Structure of the R132K:R111L:L121E Mutant of Apo-Cellular Retinoic Acid Binding Protein Type II At 1.50 Angstroms Resolution

Released:
DOI: 10.2210/pdb3d97/pdb
PDB entry 3d97 coloured by chain, front view.
PDB entry 3d97 coloured by chain, side view.
PDB entry 3d97 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Determining Crystal Structures of Proteins and Protein Complexes by X-Ray Crystallography: X-Ray Crystallographic Studies of the Mutants of Cellular Retinoic Acid Binding Protein Type II Toward Designing a Mimic of Rhodopsin.
Vaezeslami S, Jia X, Vasileiou C, Borhan B, Geiger JH
To be published

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular retinoic acid-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 137 amino acids
Theoretical weight: 15.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29373 (Residues: 2-138; Coverage: 99%)
Gene name: CRABP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand B3P 1 x B3P
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P1
Unit cell:
a: 34.553Å b: 37.22Å c: 61.058Å
α: 73.4° β: 73.7° γ: 89.73°
R-values:
R R work R free
0.161 0.154 0.227
Expression system: Escherichia coli

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