› 3d9b

X-ray diffraction
3.42Å resolution

Symmetric structure of E. coli AcrB

Released:
DOI: 10.2210/pdb3d9b/pdb
PDB entry 3d9b coloured by chain, front view.
PDB entry 3d9b coloured by chain, side view.
PDB entry 3d9b coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
There is a baby in the bath water: AcrB contamination is a major problem in membrane-protein crystallization.
Veesler D, Blangy S, Cambillau C, Sciara G
Acta Crystallogr Sect F Struct Biol Cryst Commun 64 880-5 (2008)
PMID: 18931428

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrB Chain: A
Molecule details ›
Chain: A
Length: 1049 amino acids
Theoretical weight: 113.67 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R32
Unit cell:
a: 145.74Å b: 145.74Å c: 514Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.291 0.289 0.355

Quick links

Citations

4 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
3 more

4 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
3 more