› 3dfr

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURES OF ESCHERICHIA COLI AND LACTOBACILLUS CASEI DIHYDROFOLATE REDUCTASE REFINED AT 1.7 ANGSTROMS RESOLUTION. I. GENERAL FEATURES AND BINDING OF METHOTREXATE

Released:
DOI: 10.2210/pdb3dfr/pdb
PDB entry 3dfr coloured by chain, front view.
PDB entry 3dfr coloured by chain, side view.
PDB entry 3dfr coloured by chain, top view.
Source organism: Lacticaseibacillus casei
Primary publication:
Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. I. General features and binding of methotrexate.
Bolin JT, Filman DJ, Matthews DA, Hamlin RC, Kraut J
J Biol Chem 257 13650-62 (1982)
PMID: 6815178

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132453 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.35 KDa
Source organism: Lacticaseibacillus casei
Expression system: Not provided
UniProt:
  • Canonical: P00381 (Residues: 2-163; Coverage: 99%)
Gene names: dhfR, folA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
1 bound ligand:
Ligand MTX 1 x MTX
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P61
Unit cell:
a: 71.86Å b: 71.86Å c: 93.38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.152 0.152 not available
Expression system: Not provided

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Citations

26 review citations

Hydrogen bonding in globular proteins.
Baker et al. (1984)
25 more

36 mentions without citation

What lessons can be learned from studying the folding of homologous proteins?
Nickson et al. (2010)
35 more