› 3dhw

X-ray diffraction
3.7Å resolution

Crystal structure of methionine importer MetNI

Released:
DOI: 10.2210/pdb3dhw/pdb
PDB entry 3dhw coloured by chain, front view.
PDB entry 3dhw coloured by chain, side view.
PDB entry 3dhw coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation.
Kadaba NS, Kaiser JT, Johnson E, Lee A, Rees DC
Science 321 250-3 (2008)
PMID: 18621668

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + D-methionine-[methionine-binding protein](Side 1) = ADP + phosphate + D-methionine(Side 2) + [methionine-binding protein](Side 1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151799 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
D-methionine transport system permease protein MetI Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 217 amino acids
Theoretical weight: 23.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31547 (Residues: 1-217; Coverage: 100%)
Gene names: JW0194, b0198, metI, yaeE
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like
Methionine import ATP-binding protein MetN Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 343 amino acids
Theoretical weight: 37.83 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P30750 (Residues: 1-343; Coverage: 100%)
Gene names: JW0195, abc, b0199, metN
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1, SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 97.7Å b: 165.4Å c: 289.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.31 0.31 0.347
Expression system: Escherichia coli

Quick links

Citations

33 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
32 more

17 mentions without citation

ABC transporters: the power to change.
Rees et al. (2009)
16 more