› 3dlh

X-ray diffraction
3Å resolution

Crystal structure of the guide-strand-containing Argonaute protein silencing complex

Released:
DOI: 10.2210/pdb3dlh/pdb
PDB entry 3dlh coloured by chain, front view.
PDB entry 3dlh coloured by chain, side view.
PDB entry 3dlh coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structure of the guide-strand-containing argonaute silencing complex.
Wang Y, Sheng G, Juranek S, Tuschl T, Patel DJ
Nature 456 209-13 (2008)
PMID: 18754009

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116178 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protein argonaute Chains: A, B
Molecule details ›
Chains: A, B
Length: 685 amino acids
Theoretical weight: 76.73 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746M7 (Residues: 1-685; Coverage: 100%)
Gene names: TT_P0026, ago
Sequence domains:
Structure domains:
DNA (5'-D(DTP*DGP*DAP*DGP*DGP*DTP*DAP*DGP*DTP*DAP*DGP*DGP*DTP*DTP*DGP*DTP*DAP*DTP*DAP*DGP*DT)-3') Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 21 nucleotides
Theoretical weight: 6.59 KDa
Source organism: Thermus thermophilus HB27
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand MG 3 x MG
Ligand PGE 1 x PGE
Ligand PO4 2 x PO4
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 74.744Å b: 114.629Å c: 175.689Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.284
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

78 review citations

Origins and Mechanisms of miRNAs and siRNAs.
Carthew et al. (2009)
77 more

25 mentions without citation

A parsimonious model for gene regulation by miRNAs.
Djuranovic et al. (2011)
24 more