› 3dlz

X-ray diffraction
1.85Å resolution

Crystal structure of human haspin in complex with AMP

Released:
DOI: 10.2210/pdb3dlz/pdb
PDB entry 3dlz coloured by chain, front view.
PDB entry 3dlz coloured by chain, side view.
PDB entry 3dlz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and functional characterization of the atypical human kinase haspin.
Eswaran J, Patnaik D, Filippakopoulos P, Wang F, Stein RL, Murray JW, Higgins JM, Knapp S
Proc Natl Acad Sci U S A 106 20198-203 (2009)
PMID: 19918057

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase haspin Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TF76 (Residues: 465-798; Coverage: 42%)
Gene names: GSG2, HASPIN
Sequence domains: Haspin like kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand AMP 1 x AMP
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 73.315Å b: 49.729Å c: 98.009Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.164 0.216
Expression system: Escherichia coli

Quick links

Citations

20 review citations

The structural basis for control of eukaryotic protein kinases.
Endicott et al. (2012)
19 more

8 mentions without citation

The Ins and Outs of Aurora B Inner Centromere Localization.
Hindriksen et al. (2017)
7 more