› 3dm3

X-ray diffraction
2.4Å resolution

Crystal structure of a domain of a Replication factor A protein, from Methanocaldococcus jannaschii. NorthEast Structural Genomics target MjR118E

Released:
DOI: 10.2210/pdb3dm3/pdb
PDB entry 3dm3 coloured by chain, front view.
PDB entry 3dm3 coloured by chain, side view.
PDB entry 3dm3 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Entry authors: Seetharaman J, Su M, Maglaqui M, Janjua H, Ciccosanti C, Xiao R, Nair R, Everett JK, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176645 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replication factor A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 105 amino acids
Theoretical weight: 11.74 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58559 (Residues: 170-274; Coverage: 16%)
Gene names: MJ1159, rpa
Sequence domains: OB-fold nucleic acid binding domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

1 bound ligand:
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P6522
Unit cell:
a: 76.001Å b: 76.001Å c: 249.479Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.274
Expression system: Escherichia coli

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