› 3dmm

X-ray diffraction
2.6Å resolution

Crystal structure of the CD8 alpha beta/H-2Dd complex

Released:
DOI: 10.2210/pdb3dmm/pdb
PDB entry 3dmm coloured by chain, front view.
PDB entry 3dmm coloured by chain, side view.
PDB entry 3dmm coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of the CD8 alpha beta/MHC class I interaction: focused recognition orients CD8 beta to a T cell proximal position.
Wang R, Natarajan K, Margulies DH
J Immunol 183 2554-64 (2009)
PMID: 19625641

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134569 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
H-2 class I histocompatibility antigen, D-D alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 32.01 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01900 (Residues: 26-299; Coverage: 80%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Synthetic peptide Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 1.08 KDa
T-cell surface glycoprotein CD8 alpha chain Chain: C
Molecule details ›
Chain: C
Length: 166 amino acids
Theoretical weight: 18.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01731 (Residues: 23-188; Coverage: 73%)
Gene names: Cd8a, Lyt-2, Lyt2
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
T-cell surface glycoprotein CD8 beta chain Chain: D
Molecule details ›
Chain: D
Length: 150 amino acids
Theoretical weight: 16.88 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10300 (Residues: 19-168; Coverage: 77%)
Gene names: Cd8b, Cd8b1, Ly-3, Lyt-3, Lyt3
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 79.468Å b: 96.694Å c: 97.544Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.292
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The structural basis of αβ T-lineage immune recognition: TCR docking topologies, mechanotransduction, and co-receptor function.
Wang et al. (2012)
8 more

18 mentions without citation

Strict Major Histocompatibility Complex Molecule Class-Specific Binding by Co-Receptors Enforces MHC-Restricted αβ TCR Recognition during T Lineage Subset Commitment.
Li et al. (2013)
17 more