› 3dnj

X-ray diffraction
1.15Å resolution

The structure of the Caulobacter crescentus ClpS protease adaptor protein in complex with a N-end rule peptide

Released:
DOI: 10.2210/pdb3dnj/pdb
PDB entry 3dnj coloured by chain, front view.
PDB entry 3dnj coloured by chain, side view.
PDB entry 3dnj coloured by chain, top view.
Source organism: Caulobacter vibrioides
Primary publication:
The molecular basis of N-end rule recognition.
Wang KH, Roman-Hernandez G, Grant RA, Sauer RT, Baker TA
Mol Cell 32 406-14 (2008)
PMID: 18995838

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163490 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-dependent Clp protease adapter protein ClpS Chains: A, B
Molecule details ›
Chains: A, B
Length: 85 amino acids
Theoretical weight: 9.94 KDa
Source organism: Caulobacter vibrioides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9A5I0 (Residues: 35-119; Coverage: 71%)
Gene names: CC_2467, clpS
Sequence domains: ATP-dependent Clp protease adaptor protein ClpS
Structure domains: Ribosomal Protein L30; Chain: A,
synthetic N-end rule peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 amino acids
Theoretical weight: 1.29 KDa
Source organism: Caulobacter vibrioides
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 33.693Å b: 53.974Å c: 44.824Å
α: 90° β: 110.39° γ: 90°
R-values:
R R work R free
0.136 0.135 0.159
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

AAA+ proteases: ATP-fueled machines of protein destruction.
Sauer et al. (2011)
15 more

13 mentions without citation

How the ends signal the end: Regulation by E3 ubiquitin ligases recognizing protein termini.
Sherpa et al. (2022)
12 more