› 3dou

X-ray diffraction
1.45Å resolution

Crystal structure of methyltransferase involved in cell division from thermoplasma volcanicum gss1

Released:
DOI: 10.2210/pdb3dou/pdb
PDB entry 3dou coloured by chain, front view.
PDB entry 3dou coloured by chain, side view.
PDB entry 3dou coloured by chain, top view.
Source organism: Thermoplasma volcanium
Entry authors: Patskovsky Y, Ozyurt S, Dickey M, Hu S, Bain K, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA large subunit methyltransferase E Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 22.1 KDa
Source organism: Thermoplasma volcanium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97C13 (Residues: 17-196; Coverage: 91%)
Gene names: TV0292, TVG0303954, rlmE, rrmJ
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 64.62Å b: 50.053Å c: 56.457Å
α: 90° β: 108.08° γ: 90°
R-values:
R R work R free
0.167 0.166 0.195
Expression system: Escherichia coli

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