› 3dpi

X-ray diffraction
2.2Å resolution

Crystal Structure of NAD+ synthetase from Burkholderia Pseudomallei

Released:
DOI: 10.2210/pdb3dpi/pdb
PDB entry 3dpi coloured by chain, front view.
PDB entry 3dpi coloured by chain, side view.
PDB entry 3dpi coloured by chain, top view.
Source organism: Burkholderia pseudomallei 1710b
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 31.01 KDa
Source organism: Burkholderia pseudomallei 1710b
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JL79 (Residues: 1-284; Coverage: 100%)
Gene names: BURPS1710b_A0515, nadE
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P41212
Unit cell:
a: 76.315Å b: 76.315Å c: 183.772Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.248
Expression system: Escherichia coli

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