› 3dqb

X-ray diffraction
3.2Å resolution

Crystal structure of the active G-protein-coupled receptor opsin in complex with a C-terminal peptide derived from the Galpha subunit of transducin

Released:
DOI: 10.2210/pdb3dqb/pdb
PDB entry 3dqb coloured by chain, front view.
PDB entry 3dqb coloured by chain, side view.
PDB entry 3dqb coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of opsin in its G-protein-interacting conformation.
Scheerer P, Park JH, Hildebrand PW, Kim YJ, Krauss N, Choe HW, Hofmann KP, Ernst OP
Nature 455 497-502 (2008)
PMID: 18818650

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136066 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins
Guanine nucleotide-binding protein G(t) subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.27 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P04695 (Residues: 340-350; Coverage: 3%)
Gene name: GNAT1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand BOG 3 x BOG
Ligand PLM 2 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: R32
Unit cell:
a: 238.02Å b: 238.02Å c: 109.132Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.213 0.248
Expression system: Not provided

Quick links

Citations

160 review citations

The structure and function of G-protein-coupled receptors.
Rosenbaum et al. (2009)
159 more

99 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
98 more