› 3dsd

X-ray diffraction
2.2Å resolution

Crystal structure of P. furiosus Mre11-H85S bound to a branched DNA and manganese

Released:
DOI: 10.2210/pdb3dsd/pdb
PDB entry 3dsd coloured by chain, front view.
PDB entry 3dsd coloured by chain, side view.
PDB entry 3dsd coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Mre11 dimers coordinate DNA end bridging and nuclease processing in double-strand-break repair.
Williams RS, Moncalian G, Williams JS, Yamada Y, Limbo O, Shin DS, Groocock LM, Cahill D, Hitomi C, Guenther G, Moiani D, Carney JP, Russell P, Tainer JA
Cell 135 97-109 (2008)
PMID: 18854158

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116185 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA double-strand break repair protein Mre11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 40.87 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U1N9 (Residues: 1-343; Coverage: 81%)
Gene names: PF1166, mre11
Sequence domains: Calcineurin-like phosphoesterase
Structure domains:
DNA (5'-D(*DCP*DGP*DCP*DGP*DCP*DAP*DCP*DAP*DAP*DGP*DCP*DTP*DTP*DTP*DTP*DGP*DCP*DTP*DTP*DGP*DTP*DGP*DGP*DAP*DTP*DA)-3') Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 7.99 KDa
Source organism: Pyrococcus furiosus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P212121
Unit cell:
a: 77.157Å b: 88.128Å c: 137.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.246
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

108 review citations

Double-strand break end resection and repair pathway choice.
Symington et al. (2011)
107 more

17 mentions without citation

Repair of double-strand breaks by end joining.
Chiruvella et al. (2013)
16 more