› 3dto

X-ray diffraction
3.3Å resolution

Crystal structure of the metal-dependent HD domain-containing hydrolase BH2835 from Bacillus halodurans, Northeast Structural Genomics Consortium Target BhR130.

Released:
DOI: 10.2210/pdb3dto/pdb
PDB entry 3dto coloured by chain, front view.
PDB entry 3dto coloured by chain, side view.
PDB entry 3dto coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Entry authors: Forouhar F, Su M, Seetharaman J, Janjua H, Fang Y, Xiao R, Cunningham K, Ma L-C, Owen LA, Wang D, Tong S, Everett JK, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HD/PDEase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 223 amino acids
Theoretical weight: 26.2 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K916 (Residues: 1-215; Coverage: 100%)
Gene name: BH2835
Sequence domains: HD domain
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 97.564Å b: 47.987Å c: 111.159Å
α: 90° β: 89.99° γ: 90°
R-values:
R R work R free
0.248 0.248 0.292
Expression system: Escherichia coli

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