› 3du7

X-ray diffraction
4.1Å resolution

Tubulin-colchicine-phomopsin A: Stathmin-like domain complex

Released:
DOI: 10.2210/pdb3du7/pdb
PDB entry 3du7 coloured by chain, front view.
PDB entry 3du7 coloured by chain, side view.
PDB entry 3du7 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into the inhibition of tubulin by vinca domain peptide ligands.
Cormier A, Marchand M, Ravelli RB, Knossow M, Gigant B
EMBO Rep 9 1101-6 (2008)
PMID: 18787557

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-158933 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1C chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 449 amino acids
Theoretical weight: 50.09 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3ZCJ7 (Residues: 1-449; Coverage: 100%)
Gene name: TUBA1C
Sequence domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 49.97 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 142 amino acids
Theoretical weight: 16.71 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand CN2 2 x CN2
Ligand HOS 2 x HOS
Ligand GTP 2 x GTP
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P65
Unit cell:
a: 327.126Å b: 327.126Å c: 53.672Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.265
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Microtubule-binding agents: a dynamic field of cancer therapeutics.
Dumontet et al. (2010)
9 more

8 mentions without citation

Super-resolution biomolecular crystallography with low-resolution data.
Schröder et al. (2010)
7 more