› 3dvl

X-ray diffraction
2.8Å resolution

Crystal Structure of Full Length Circadian Clock Protein KaiC with Correct Geometry at Phosphorylation Sites

Released:
DOI: 10.2210/pdb3dvl/pdb
PDB entry 3dvl coloured by chain, front view.
PDB entry 3dvl coloured by chain, side view.
PDB entry 3dvl coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Entry authors: Pattanayek R, Egli M

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Circadian clock oscillator protein KaiC Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 519 amino acids
Theoretical weight: 58.23 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli
UniProt:
  • Canonical: Q79PF4 (Residues: 1-519; Coverage: 100%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 6 x MG
Ligand ATP 12 x ATP
2 modified residues:
Ligand SEP 6 x SEP
Ligand TPO 6 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID, APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 132.873Å b: 135.576Å c: 204.951Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.288
Expression system: Escherichia coli

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