3dw9

X-ray diffraction
2.2Å resolution

SgrAI with cognate DNA and manganese bound

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-116187 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
SgraIR restriction enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 37.94 KDa
Source organism: Streptomyces griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F6L0 (Residues: 2-339; Coverage: 100%)
Gene name: sgraIR
Sequence domains: Cfr10I/Bse634I restriction endonuclease
Structure domains: Restriction Endonuclease
DNA (5'-D(*DGP*DAP*DGP*DTP*DCP*DCP*DAP*DCP*DCP*DGP*DGP*DTP*DGP*DGP*DAP*DCP*DTP*DC)-3') Chains: E, F
Molecule details ›
Chains: E, F
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Streptomyces griseus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 58.261Å b: 117.101Å c: 63.702Å
α: 90° β: 112.96° γ: 90°
R-values:
R R work R free
0.177 0.174 0.24
Expression systems:
  • Escherichia coli
  • Not provided