› 3dwa

X-ray diffraction
2.08Å resolution

Crystal structure of the B-subunit of the AB5 toxin from E. coli

Released:
DOI: 10.2210/pdb3dwa/pdb
PDB entry 3dwa coloured by chain, front view.
PDB entry 3dwa coloured by chain, side view.
PDB entry 3dwa coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Incorporation of a non-human glycan mediates human susceptibility to a bacterial toxin.
Byres E, Paton AW, Paton JC, Löfling JC, Smith DF, Wilce MC, Talbot UM, Chong DC, Yu H, Huang S, Chen X, Varki NM, Varki A, Rossjohn J, Beddoe T
Nature 456 648-52 (2008)
PMID: 18971931

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Subtilase SubB Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 126 amino acids
Theoretical weight: 14.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3ZTX8 (Residues: 24-141; Coverage: 100%)
Gene names: LH0182, subB
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:
Ligand 1PE 5 x 1PE
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P65
Unit cell:
a: 97.63Å b: 97.63Å c: 165.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.189 0.236
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Sialic acids as regulators of molecular and cellular interactions.
Schauer R. (2009)
40 more

5 mentions without citation

Incorporation of a non-human glycan mediates human susceptibility to a bacterial toxin.
Byres et al. (2008)
4 more