› 3dwf

X-ray diffraction
2.2Å resolution

Crystal Structure of the Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1 Mutant F278E

Released:
DOI: 10.2210/pdb3dwf/pdb
PDB entry 3dwf coloured by chain, front view.
PDB entry 3dwf coloured by chain, side view.
PDB entry 3dwf coloured by chain, top view.
Source organism: Cavia porcellus
Primary publication:
Mutations of key hydrophobic surface residues of 11 beta-hydroxysteroid dehydrogenase type 1 increase solubility and monodispersity in a bacterial expression system.
Lawson AJ, Walker EA, White SA, Dafforn TR, Stewart PM, Ride JP
Protein Sci 18 1552-63 (2009)
PMID: 19507261

Function and Biology Details

Reactions catalysed: 
An 11-beta-hydroxysteroid + NADP(+) = an 11-oxosteroid + NADPH
A 7-beta-hydroxysteroid + NADP(+) = a 7-oxosteroid + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180271 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
11-beta-hydroxysteroid dehydrogenase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 276 amino acids
Theoretical weight: 30.36 KDa
Source organism: Cavia porcellus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6QLL4 (Residues: 24-299; Coverage: 92%)
Gene name: HSD11B1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
2 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 78.08Å b: 85.92Å c: 176.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.255
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Cortisone-reductase deficiency associated with heterozygous mutations in 11beta-hydroxysteroid dehydrogenase type 1.
Lawson et al. (2011)
6 more

2 mentions without citation

Crystal structures of 11β-hydroxysteroid dehydrogenase type 1 and their use in drug discovery.
Thomas et al. (2011)
1 more