› 3dwp

X-ray diffraction
2.2Å resolution

Crystal structure of the B-subunit of the AB5 toxin from E. Coli with Neu5Gc

Released:
DOI: 10.2210/pdb3dwp/pdb
PDB entry 3dwp coloured by chain, front view.
PDB entry 3dwp coloured by chain, side view.
PDB entry 3dwp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Incorporation of a non-human glycan mediates human susceptibility to a bacterial toxin.
Byres E, Paton AW, Paton JC, Löfling JC, Smith DF, Wilce MC, Talbot UM, Chong DC, Yu H, Huang S, Chen X, Varki NM, Varki A, Rossjohn J, Beddoe T
Nature 456 648-52 (2008)
PMID: 18971931

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Subtilase SubB Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 126 amino acids
Theoretical weight: 14.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3ZTX8 (Residues: 24-141; Coverage: 100%)
Gene names: LH0182, subB
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand NGC 5 x NGC
Ligand 1PE 5 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P65
Unit cell:
a: 96.9Å b: 96.9Å c: 165.622Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.188 0.24
Expression system: Escherichia coli

Quick links

Citations

40 review citations

Sialic acids as regulators of molecular and cellular interactions.
Schauer R. (2009)
39 more

6 mentions without citation

Protein Toxins That Utilize Gangliosides as Host Receptors.
Zuverink et al. (2018)
5 more