› 3dwv

X-ray diffraction
1.41Å resolution

Glutathione peroxidase-type tryparedoxin peroxidase, oxidized form

Released:
DOI: 10.2210/pdb3dwv/pdb
PDB entry 3dwv coloured by chain, front view.
PDB entry 3dwv coloured by chain, side view.
PDB entry 3dwv coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
Structural basis for a distinct catalytic mechanism in Trypanosoma brucei tryparedoxin peroxidase.
Melchers J, Diechtierow M, Fehér K, Sinning I, Tews I, Krauth-Siegel RL, Muhle-Goll C
J Biol Chem 283 30401-11 (2008)
PMID: 18684708

Function and Biology Details

Reaction catalysed: 
2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione peroxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 187 amino acids
Theoretical weight: 20.98 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q869A5 (Residues: 2-176; Coverage: 99%)
Gene name: gpx3
Sequence domains: Glutathione peroxidase
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 42.81Å b: 105.864Å c: 42.738Å
α: 90° β: 100.48° γ: 90°
R-values:
R R work R free
0.128 0.116 0.176
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes.
Deponte M. (2013)
3 more

5 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
4 more