› 3dx0

X-ray diffraction
1.7Å resolution

Golgi alpha-Mannosidase II in complex with Mannostatin A at pH 5.75

Released:
DOI: 10.2210/pdb3dx0/pdb
PDB entry 3dx0 coloured by chain, front view.
PDB entry 3dx0 coloured by chain, side view.
PDB entry 3dx0 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The molecular basis of inhibition of Golgi alpha-mannosidase II by mannostatin A.
Kuntz DA, Zhong W, Guo J, Rose DR, Boons GJ
Chembiochem 10 268-77 (2009)
PMID: 19101978

Function and Biology Details

Reaction catalysed: 
Man(5)GlcNAc(3)-[protein] + 2 H(2)O = Man(3)GlcNAc(3)-[protein] + 2 alpha-D-mannopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

5 bound ligands:
Ligand NAG 1 x NAG
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
Ligand MSN 1 x MSN
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 68.853Å b: 109.86Å c: 139.136Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.16 0.186
Expression system: Drosophila melanogaster

Quick links

Citations

2 review citations

Structure, mechanism and inhibition of Golgi α-mannosidase II.
Rose DR. (2012)
1 more

2 mentions without citation

The Role of Proteolysis in Amyloidosis.
Acquasaliente et al. (2022)
1 more