› 3dy5

X-ray diffraction
3.51Å resolution

Allene oxide synthase 8R-lipoxygenase from Plexaura homomalla

Released:
DOI: 10.2210/pdb3dy5/pdb
Model geometry
Fit model/data
PDB entry 3dy5 coloured by chain, front view.
PDB entry 3dy5 coloured by chain, side view.
PDB entry 3dy5 coloured by chain, top view.
Source organism: Plexaura homomalla
Primary publication:
A covalent linker allows for membrane targeting of an oxylipin biosynthetic complex.
Gilbert NC, Niebuhr M, Tsuruta H, Bordelon T, Ridderbusch O, Dassey A, Brash AR, Bartlett SG, Newcomer ME
Biochemistry 47 10665-76 (2008)
PMID: 18785758

Function and Biology Details

Reaction catalysed: 
Arachidonate + O(2) = (5Z,9E,11Z,14Z)-(8R)-8-hydroperoxyicosa-5,9,11,14-tetraenoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Allene oxide synthase-lipoxygenase protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 1066 amino acids
Theoretical weight: 121.97 KDa
Source organism: Plexaura homomalla
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O16025 (Residues: 1-1066; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FE2 2 x FE2
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CAMD BEAMLINE GCPCC
Spacegroup: C2
Unit cell:
a: 235.52Å b: 77.493Å c: 157.966Å
α: 90° β: 112.45° γ: 90°
R-values:
R R work R free
0.275 0.273 0.322
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

ALLENE OXIDE SYNTHASE and HYDROPEROXIDE LYASE, Two Non-Canonical Cytochrome P450s in Arabidopsis thaliana and Their Different Roles in Plant Defense.
Rustgi et al. (2019)
4 other articles

4 mentions without citation

5-(4-Hydroxy-2,3,5-trimethylbenzylidene) thiazolidine-2,4-dione attenuates atherosclerosis possibly by reducing monocyte recruitment to the lesion.
Choi et al. (2011)
3 more