› 3dzc

X-ray diffraction
2.35Å resolution

2.35 Angstrom resolution structure of WecB (VC0917), a UDP-N-acetylglucosamine 2-epimerase from Vibrio cholerae.

Released:
DOI: 10.2210/pdb3dzc/pdb
PDB entry 3dzc coloured by chain, front view.
PDB entry 3dzc coloured by chain, side view.
PDB entry 3dzc coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Minasov G, Shuvalova L, Dubrovska I, Winsor J, Papazisi L, Kwon K, Hasseman J, Peterson SN, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192090 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing) Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 43.82 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KTI5 (Residues: 1-372; Coverage: 100%)
Gene name: VC_0917
Sequence domains: UDP-N-acetylglucosamine 2-epimerase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 81.391Å b: 88.127Å c: 132.193Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.172 0.234
Expression system: Escherichia coli

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