› 3dzd

X-ray diffraction
2.4Å resolution

Crystal structure of sigma54 activator NTRC4 in the inactive state

Released:
DOI: 10.2210/pdb3dzd/pdb
PDB entry 3dzd coloured by chain, front view.
PDB entry 3dzd coloured by chain, side view.
PDB entry 3dzd coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structure and regulatory mechanism of Aquifex aeolicus NtrC4: variability and evolution in bacterial transcriptional regulation.
Batchelor JD, Doucleff M, Lee CJ, Matsubara K, De Carlo S, Heideker J, Lamers MH, Pelton JG, Wemmer DE
J Mol Biol 384 1058-75 (2008)
PMID: 18955063

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130341 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator (NtrC family) Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 41.95 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66551 (Residues: 2-369; Coverage: 83%)
Gene names: aq_164, ntrC4
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 89.897Å b: 93.105Å c: 114.163Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.262
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Diversity of structure and function of response regulator output domains.
Galperin MY. (2010)
10 more

29 mentions without citation

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
28 more