› 3dzv

X-ray diffraction
2.57Å resolution

Crystal structure of 4-methyl-5-(beta-hydroxyethyl)thiazole kinase (NP_816404.1) from ENTEROCOCCUS FAECALIS V583 at 2.57 A resolution

Released:
DOI: 10.2210/pdb3dzv/pdb
PDB entry 3dzv coloured by chain, front view.
PDB entry 3dzv coloured by chain, side view.
PDB entry 3dzv coloured by chain, top view.
Source organism: Enterococcus faecalis
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxyethylthiazole kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 30.13 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q830K4 (Residues: 1-272; Coverage: 100%)
Gene names: EF_2777, thiM
Sequence domains: Hydroxyethylthiazole kinase family
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand ADP 2 x ADP
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I213
Unit cell:
a: 181.705Å b: 181.705Å c: 181.705Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.204
Expression system: Escherichia coli

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