› 3e05

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF Precorrin-6y C5,15-methyltransferase FROM Geobacter metallireducens GS-15

Released:
DOI: 10.2210/pdb3e05/pdb
PDB entry 3e05 coloured by chain, front view.
PDB entry 3e05 coloured by chain, side view.
PDB entry 3e05 coloured by chain, top view.
Source organism: Geobacter metallireducens GS-15
Entry authors: Patskovsky Y, Ramagopal UA, Toro R, Dickey M, Hu S, Maletic M, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + precorrin-6B = S-adenosyl-L-homocysteine + precorrin-7 + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetrapyrrole methylase domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 204 amino acids
Theoretical weight: 22.73 KDa
Source organism: Geobacter metallireducens GS-15
Expression system: Escherichia coli
UniProt:
  • Canonical: Q39YF0 (Residues: 211-405; Coverage: 48%)
Gene names: Gmet_0481, cbiET
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand CL 8 x CL
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 124.71Å b: 83.451Å c: 149.056Å
α: 90° β: 95.72° γ: 90°
R-values:
R R work R free
0.217 0.216 0.262
Expression system: Escherichia coli

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