› 3e0u

X-ray diffraction
2.3Å resolution

Crystal structure of T. cruzi GPX1

Released:
DOI: 10.2210/pdb3e0u/pdb
PDB entry 3e0u coloured by chain, front view.
PDB entry 3e0u coloured by chain, side view.
PDB entry 3e0u coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Structural insights into the catalytic mechanism of Trypanosoma cruzi GPXI (glutathione peroxidase-like enzyme I).
Patel S, Hussain S, Harris R, Sardiwal S, Kelly JM, Wilkinson SR, Driscoll PC, Djordjevic S
Biochem J 425 513-22 (2010)
PMID: 19886864

Function and Biology Details

Reaction catalysed: 
2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione peroxidase Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.28 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8T8E2 (Residues: 14-177; Coverage: 93%)
Gene name: GPXI
Sequence domains: Glutathione peroxidase
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand NH4 1 x NH4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 73.846Å b: 46.591Å c: 61.163Å
α: 90° β: 121.54° γ: 90°
R-values:
R R work R free
0.21 0.207 0.254
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Redox Balance Keepers and Possible Cell Functions Managed by Redox Homeostasis in Trypanosoma cruzi.
Mesías et al. (2019)
1 more

2 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
1 more