3e1y

X-ray diffraction
3.8Å resolution

Crystal structure of human eRF1/eRF3 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-147260 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic peptide chain release factor subunit 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 451 amino acids
Theoretical weight: 50.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62495 (Residues: 1-437; Coverage: 100%)
Gene names: ERF1, ETF1, RF1, SUP45L1
Sequence domains:
Structure domains:
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 204 amino acids
Theoretical weight: 22.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15170 (Residues: 301-499; Coverage: 40%)
Gene names: ERF3A, GSPT1
Sequence domains:
Structure domains: Translation factors

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43
Unit cell:
a: 173.966Å b: 173.966Å c: 119.824Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.26 0.304
Expression system: Escherichia coli