› 3e20

X-ray diffraction
3.5Å resolution

Crystal structure of S.pombe eRF1/eRF3 complex

Released:
DOI: 10.2210/pdb3e20/pdb
PDB entry 3e20 coloured by chain, front view.
PDB entry 3e20 coloured by chain, side view.
PDB entry 3e20 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Structural insights into eRF3 and stop codon recognition by eRF1.
Cheng Z, Saito K, Pisarev AV, Wada M, Pisareva VP, Pestova TV, Gajda M, Round A, Kong C, Lim M, Nakamura Y, Svergun DI, Ito K, Song H
Genes Dev 23 1106-18 (2009)
PMID: 19417105

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130794 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic peptide chain release factor GTP-binding subunit Chains: A, D, E, J
Molecule details ›
Chains: A, D, E, J
Length: 201 amino acids
Theoretical weight: 22.21 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O74718 (Residues: 467-662; Coverage: 30%)
Gene names: SPCC584.04, sup35
Sequence domains: Elongation factor Tu C-terminal domain
Structure domains: Translation factors
Eukaryotic peptide chain release factor subunit 1 Chains: B, C, H, K
Molecule details ›
Chains: B, C, H, K
Length: 441 amino acids
Theoretical weight: 49.95 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: P79063 (Residues: 1-433; Coverage: 100%)
Gene names: SPAC1834.01, sup45
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P43
Unit cell:
a: 129.848Å b: 129.848Å c: 332.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.258 0.28
Expression system: Escherichia coli

Quick links

Citations

22 review citations

NMD: a multifaceted response to premature translational termination.
Kervestin et al. (2012)
21 more

2 mentions without citation

Structural insights into eRF3 and stop codon recognition by eRF1.
Cheng et al. (2009)
1 more