› 3e22

X-ray diffraction
3.8Å resolution

Tubulin-colchicine-soblidotin: Stathmin-like domain complex

Released:
DOI: 10.2210/pdb3e22/pdb
PDB entry 3e22 coloured by chain, front view.
PDB entry 3e22 coloured by chain, side view.
PDB entry 3e22 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into the inhibition of tubulin by vinca domain peptide ligands.
Cormier A, Marchand M, Ravelli RB, Knossow M, Gigant B
EMBO Rep 9 1101-6 (2008)
PMID: 18787557

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-158933 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1C chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 449 amino acids
Theoretical weight: 50.09 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3ZCJ7 (Residues: 1-449; Coverage: 100%)
Gene name: TUBA1C
Sequence domains:
Structure domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 49.97 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Structure domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 142 amino acids
Theoretical weight: 16.71 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

5 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand GDP 2 x GDP
Ligand LOC 2 x LOC
Ligand TZT 1 x TZT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P65
Unit cell:
a: 324.093Å b: 324.093Å c: 53.28Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.229 0.295
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Microtubule-binding agents: a dynamic field of cancer therapeutics.
Dumontet et al. (2010)
8 more

13 mentions without citation

Targeting and extending the eukaryotic druggable genome with natural products: cytoskeletal targets of natural products.
Risinger et al. (2020)
12 more