› 3e2d

X-ray diffraction
1.4Å resolution

The 1.4 A crystal structure of the large and cold-active Vibrio sp. alkaline phosphatase

Released:
DOI: 10.2210/pdb3e2d/pdb
PDB entry 3e2d coloured by chain, front view.
PDB entry 3e2d coloured by chain, side view.
PDB entry 3e2d coloured by chain, top view.
Source organism: Vibrio sp. G15-21
Primary publication:
The 1.4 A crystal structure of the large and cold-active Vibrio sp. alkaline phosphatase.
Helland R, Larsen RL, Asgeirsson B
Biochim Biophys Acta 1794 297-308 (2009)
PMID: 18977465

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 502 amino acids
Theoretical weight: 55.43 KDa
Source organism: Vibrio sp. G15-21
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93P54 (Residues: 20-521; Coverage: 96%)
Sequence domains: Alkaline phosphatase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand MG 2 x MG
Ligand SO4 11 x SO4
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21212
Unit cell:
a: 118.24Å b: 165.98Å c: 57.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.166
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Cellular function and molecular structure of ecto-nucleotidases.
Zimmermann et al. (2012)
1 more

11 mentions without citation

Psychrophilic enzymes: from folding to function and biotechnology.
Feller G. (2013)
10 more