› 3e48

X-ray diffraction
1.6Å resolution

Crystal structure of a nucleoside-diphosphate-sugar epimerase (SAV0421) from Staphylococcus aureus, Northeast Structural Genomics Consortium Target ZR319

Released:
DOI: 10.2210/pdb3e48/pdb
PDB entry 3e48 coloured by chain, front view.
PDB entry 3e48 coloured by chain, side view.
PDB entry 3e48 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Entry authors: Forouhar F, Abashidze M, Seetharaman J, Mao L, Janjua H, Xiao R, Ciccosanti C, Foote EL, Wang D, Tong S, Everett JK, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101964 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 33.57 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3JTC8 (Residues: 1-281; Coverage: 100%)
Gene name: SAV0421
Sequence domains: NAD(P)H-binding
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 65.086Å b: 67.188Å c: 141.582Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.205
Expression system: Escherichia coli

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