› 3e5c

X-ray diffraction
2.25Å resolution

Crystal Structure of the SMK box (SAM-III) Riboswitch with SAM

Released:
DOI: 10.2210/pdb3e5c/pdb
PDB entry 3e5c coloured by chain, front view.
PDB entry 3e5c coloured by chain, side view.
PDB entry 3e5c coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystal structures of the SAM-III/S(MK) riboswitch reveal the SAM-dependent translation inhibition mechanism.
Lu C, Smith AM, Fuchs RT, Ding F, Rajashankar K, Henkin TM, Ke A
Nat Struct Mol Biol 15 1076-83 (2008)
PMID: 18806797

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196659 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
SMK box (SAM-III) Riboswitch Chain: A
Molecule details ›
Chain: A
Length: 53 nucleotides
Theoretical weight: 17.4 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SR 15 x SR
Ligand SAM 1 x SAM
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E, CHESS BEAMLINE F1
Spacegroup: I4122
Unit cell:
a: 97.806Å b: 97.806Å c: 87.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.227
Expression system: Not provided

Quick links

Citations

23 review citations

A decade of riboswitches.
Serganov et al. (2013)
22 more

24 mentions without citation

The structural and functional diversity of metabolite-binding riboswitches.
Roth et al. (2009)
23 more