› 3e5e

X-ray diffraction
2.9Å resolution

Crystal Structures of the SMK box (SAM-III) Riboswitch with SAH

Released:
DOI: 10.2210/pdb3e5e/pdb
PDB entry 3e5e coloured by chain, front view.
PDB entry 3e5e coloured by chain, side view.
PDB entry 3e5e coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystal structures of the SAM-III/S(MK) riboswitch reveal the SAM-dependent translation inhibition mechanism.
Lu C, Smith AM, Fuchs RT, Ding F, Rajashankar K, Henkin TM, Ke A
Nat Struct Mol Biol 15 1076-83 (2008)
PMID: 18806797

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196660 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
SMK box (SAM-III) Riboswitch for RNA Chain: A
Molecule details ›
Chain: A
Length: 53 nucleotides
Theoretical weight: 17.4 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SR 12 x SR
Ligand SAH 1 x SAH
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-E
Spacegroup: I4122
Unit cell:
a: 98.418Å b: 98.418Å c: 86.393Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.259
Expression system: Not provided

Quick links

Citations

30 review citations

A decade of riboswitches.
Serganov et al. (2013)
29 more

5 mentions without citation

Crystal structures of the SAM-III/S(MK) riboswitch reveal the SAM-dependent translation inhibition mechanism.
Lu et al. (2008)
4 more