› 3e5h

X-ray diffraction
1.5Å resolution

Crystal Structure of Rab28 GTPase in the Active (GppNHp-bound) Form

Released:
DOI: 10.2210/pdb3e5h/pdb
PDB entry 3e5h coloured by chain, front view.
PDB entry 3e5h coloured by chain, side view.
PDB entry 3e5h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Large nucleotide-dependent conformational change in Rab28.
Lee SH, Baek K, Dominguez R
FEBS Lett 582 4107-11 (2008)
PMID: 19026641

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-28 Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 19.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51157 (Residues: 11-184; Coverage: 79%)
Gene name: RAB28
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P41212
Unit cell:
a: 79.747Å b: 79.747Å c: 66.977Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.181
Expression system: Escherichia coli

Quick links

Citations

3 review citations

DENN domain proteins: regulators of Rab GTPases.
Marat et al. (2011)
2 more

5 mentions without citation

Rab GTPases and their interacting protein partners: Structural insights into Rab functional diversity.
Pylypenko et al. (2018)
4 more