› 3e5r

X-ray diffraction
2.3Å resolution

Crystal structure and Functional Analysis of Glyceraldehyde-3-phosphate Dehydrogenase from Oryza Sativa

Released:
DOI: 10.2210/pdb3e5r/pdb
PDB entry 3e5r coloured by chain, front view.
PDB entry 3e5r coloured by chain, side view.
PDB entry 3e5r coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Entry authors: Tien YC, Lin YH, Chang SL, Chen CJ

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170970 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic Chains: A, B, C, O
Molecule details ›
Chains: A, B, C, O
Length: 337 amino acids
Theoretical weight: 36.46 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0J8A4 (Residues: 1-337; Coverage: 100%)
Gene names: GAPC, GAPC1, GAPDH, GPC, LOC_Os08g03290, OJ1163_G08.15, Os08g0126300, OsJ_024858
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P21
Unit cell:
a: 76.445Å b: 129.558Å c: 77.578Å
α: 90° β: 117.4° γ: 90°
R-values:
R R work R free
0.199 0.199 0.239
Expression system: Escherichia coli

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