› 3e5u

X-ray diffraction
1.83Å resolution

OCPA complexed CprK (C200S)

Released:
DOI: 10.2210/pdb3e5u/pdb
PDB entry 3e5u coloured by chain, front view.
PDB entry 3e5u coloured by chain, side view.
PDB entry 3e5u coloured by chain, top view.
Source organism: Desulfitobacterium hafniense DCB-2
Primary publication:
Molecular basis of halorespiration control by CprK, a CRP-FNR type transcriptional regulator.
Levy C, Pike K, Heyes DJ, Joyce MG, Gabor K, Smidt H, van der Oost J, Leys D
Mol Microbiol 70 151-67 (2008)
PMID: 18717788

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110699 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic nucleotide-binding protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 250 amino acids
Theoretical weight: 28.38 KDa
Source organism: Desulfitobacterium hafniense DCB-2
Expression system: Escherichia coli
UniProt:
  • Canonical: B8FW11 (Residues: 1-232; Coverage: 100%)
Gene name: Dhaf_0733
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 3C4 5 x 3C4
Ligand PO4 3 x PO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 56.141Å b: 117.17Å c: 84.876Å
α: 90° β: 94.65° γ: 90°
R-values:
R R work R free
0.185 0.182 0.227
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Thiol-based redox switches and gene regulation.
Antelmann et al. (2011)
8 more

3 mentions without citation

Molecular basis of halorespiration control by CprK, a CRP-FNR type transcriptional regulator.
Levy et al. (2008)
2 more