› 3e6y

X-ray diffraction
2.5Å resolution

Structure of 14-3-3 in complex with the differentiation-inducing agent Cotylenin A

Released:
DOI: 10.2210/pdb3e6y/pdb
PDB entry 3e6y coloured by chain, front view.
PDB entry 3e6y coloured by chain, side view.
PDB entry 3e6y coloured by chain, top view.
Source organisms:
Primary publication:
A structural rationale for selective stabilization of anti-tumor interactions of 14-3-3 proteins by cotylenin A.
Ottmann C, Weyand M, Sassa T, Inoue T, Kato N, Wittinghofer A, Oecking C
J Mol Biol 386 913-9 (2009)
PMID: 19244612

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161078 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3-like protein C Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 29.4 KDa
Source organism: Nicotiana tabacum
Expression system: Escherichia coli
UniProt:
  • Canonical: P93343 (Residues: 1-260; Coverage: 100%)
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Plasma membrane H+ ATPase Chains: C, D
Molecule details ›
Chains: C, D
Length: 5 amino acids
Theoretical weight: 677 Da
Source organism: Nicotiana plumbaginifolia
Expression system: Not provided
UniProt:
  • Canonical: Q40409 (Residues: 436-440; Coverage: 1%)
Gene name: pma2

Ligands and Environments

2 bound ligands:
Ligand CW1 2 x CW1
Ligand CL 1 x CL
1 modified residue:
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 41.8Å b: 141.9Å c: 51.4Å
α: 90° β: 114.1° γ: 90°
R-values:
R R work R free
0.2 0.195 0.285
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

14-3-3 proteins as signaling integration points for cell cycle control and apoptosis.
Gardino et al. (2011)
14 more

5 mentions without citation

Protein-protein interfaces in molecular glue-induced ternary complexes: classification, characterization, and prediction.
Rui et al. (2023)
4 more