› 3e70

X-ray diffraction
1.97Å resolution

Structures and conformations in solution of the Signal Recognition Particle Receptor from the Archaeon Pyrococcus Furiosus

Released:
DOI: 10.2210/pdb3e70/pdb
PDB entry 3e70 coloured by chain, front view.
PDB entry 3e70 coloured by chain, side view.
PDB entry 3e70 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Structures of the signal recognition particle receptor from the archaeon Pyrococcus furiosus: implications for the targeting step at the membrane.
Egea PF, Tsuruta H, de Leon GP, Napetschnig J, Walter P, Stroud RM
PLoS One 3 e3619 (2008)
PMID: 18978942

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186364 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle receptor FtsY Chain: C
Molecule details ›
Chain: C
Length: 328 amino acids
Theoretical weight: 36.44 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U051 (Residues: 1-322; Coverage: 100%)
Gene names: PF1766, ftsY
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 113.122Å b: 53.034Å c: 61.26Å
α: 90° β: 107.42° γ: 90°
R-values:
R R work R free
0.234 0.228 0.263
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Archaeal Signal Recognition Particle: Present Understanding and Future Perspective.
Gupta et al. (2017)
10 other articles

2 mentions without citation

Archaea signal recognition particle shows the way.
Zwieb et al. (2010)
1 more