› 3e8b

X-ray diffraction
1.7Å resolution

Crystal Structure of the the open NaK channel- Rb+ complex

Released:
DOI: 10.2210/pdb3e8b/pdb
PDB entry 3e8b coloured by chain, front view.
PDB entry 3e8b coloured by chain, side view.
PDB entry 3e8b coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
Structural analysis of ion selectivity in the NaK channel.
Alam A, Jiang Y
Nat Struct Mol Biol 16 35-41 (2009)
PMID: 19098915

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.71 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81HW2 (Residues: 19-110; Coverage: 81%)
Gene name: BC_0669
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand MPD 4 x MPD
Ligand RB 11 x RB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I4
Unit cell:
a: 68.134Å b: 68.134Å c: 89.359Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.229
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Normal mode analysis of biomolecular structures: functional mechanisms of membrane proteins.
Bahar et al. (2010)
8 more

2 mentions without citation

Structural analysis of ion selectivity in the NaK channel.
Alam et al. (2009)
1 more