› 3e8u

X-ray diffraction
2.1Å resolution

Crystal structure and thermodynamic analysis of diagnostic Fab 106.3 complexed with BNP 5-13 (C10A) reveal basis of selective molecular recognition

Released:
DOI: 10.2210/pdb3e8u/pdb
PDB entry 3e8u coloured by chain, front view.
PDB entry 3e8u coloured by chain, side view.
PDB entry 3e8u coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure and thermodynamic analysis of diagnostic mAb 106.3 complexed with BNP 5-13 (C10A).
Longenecker KL, Ruan Q, Fry EH, Saldana SC, Brophy SE, Richardson PL, Tetin SY
Proteins 76 536-47 (2009)
PMID: 19274732

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211618 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab 106.3 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 217 amino acids
Theoretical weight: 23.39 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Fab 106.3 light chain Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.76 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
BNP peptide epitope Chain: P
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 993 Da

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: C2221
Unit cell:
a: 77.9Å b: 88.878Å c: 138.509Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.243
Expression system: Not provided

Quick links

Citations

6 citation in other articles

Coexpression of molecular chaperones to enhance functional expression of anti-BNP scFv in the cytoplasm of Escherichia coli for the detection of B-type natriuretic peptide.
Maeng et al. (2011)
5 more

4 mentions without citation

OptMAVEn--a new framework for the de novo design of antibody variable region models targeting specific antigen epitopes.
Li et al. (2014)
3 more